The attorneys of Isiah Fowler were hoping new DNA evidence would convince a Calaveras County judge to release the 14-year-old from custody Friday.
However, the judge ruled that Fowler, who is accused of killing his 8-year-old sister Leila in 2013, will remain in custody until his trial begins in May.
"I disagree with the court. I think there is a change of circumstances. It was powerful evidence," Fowler's attorney, Stephen Plesser, told FOX40.
Leila Fowler was brutally stabbed to death more than 20 times in 2013 in her Valley Springs home. Isiah, who was home alone with his sister at the time, has maintained his innocence since the beginning. He has also been in custody since the beginning.
Friday was his first chance to be released.
The new evidence is one of Leila's own hairs, covered in DNA evidence from an unknown adult male. Investigators say the DNA matches no one in the Fowler family, which is why they are currently testing other people to find a match.
Isiah's attorney tells FOX40 that DNA on that hair likely came from someone's bodily fluids. In the meantime, prosecutors believe the evidence against Isiah is strong.